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Редактором является Нора Николак Габай, контактные данные - editorial_office@biochemia-medica.com, jasna.dogic@gmail.com.
Это официальный рецензируемый журнал Хорватского общества медицинской биохимии и лабораторной медицины. Он обеспечивает широкий охват исследований во всех аспектах клинической химии и лабораторной медицины. В тематику журнала входят следующие категории: общая клиническая химия, гематология и гемостаз, молекулярная диагностика и эндокринология. Приветствуется разработка, валидация и верификация аналитических методик, применимых к клинической химии и лабораторной медицине, а также исследования, касающиеся организации лабораторий, автоматизации и контроля качества. Журнал регулярно публикует обучающие преданалитические тематические отчеты (Preanalytical mysteries), статьи, посвященные прикладной биостатистике (Lessons in biostatistics) и целостности исследований (Research integrity corner).
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Пример статьи, название - Detection of macroenzymes: establishing upper reference limits for eight enzymes after polyethylene glycol precipitation. Заголовок (Abstract)
Introduction
The presence of macroenzymes in blood can cause diagnostic confusion. Therefore, confirming the presence of macroenzymes is important to reduce unnecessary (non-)invasive investigations. Polyethylene glycol (PEG) precipitation is a simple and fast first-line method for the detection of macroenzymes. However, there is no consensus on the upper reference limit for the PEG-precipitable activity (%PPA) of monomeric enzymes. The aim of this study was to verify a PEG precipitation protocol for the detection of macroenzymes in our laboratory by establishing upper reference limits (URLs) and determining imprecision for eight enzymes after PEG precipitation. In addition, we aimed to clinically verify the URLs using samples containing macroenzymes as identified by electrophoresis.
Materials and methods
Per enzyme, at least 40 leftover blood samples from adult patients with either normal or increased enzyme activities were diluted 1:1 with 25% PEG 6000 and 1:1 with 0.9% NaCl. Mixtures were incubated for 10 min at 37°C and centrifuged. Supernatant enzyme activity was measured on Cobas c702 and the %PPA was calculated.
Results
The following URLs were obtained: 26% PPA for amylase, 29% PPA for alkaline phosphatase (ALP), 61% PPA for alanine aminotransferase, 48% PPA for aspartate aminotransferase, 24% PPA for creatine kinase (CK), 55% PPA for gamma-glutamyltransferase, 65% PPA for lactate dehydrogenase, and 56% PPA for lipase. The within-lab imprecision was < 15%. Regarding the clinical verification, the two historical samples with proven macroCK showed a %PPA of 69% and 43%, respectively, and a sample with proven macroALP had a %PPA of 52%.
Conclusion
In this study, URLs for monomeric enzyme activities after PEG precipitation for eight different enzymes were established. The URLs are suitable for clinical use, but are only partially in line with other studies. Therefore, our data highlight the importance of establishing laboratory-specific upper reference limits for %PPA to allow a correct interpretation.
Keywords: precipitation; enzymes; macroamylase; polyethylene glycol; reference value
